wnt signaling pathway inhibitor xav939  (MedChemExpress)

Journal: Molecular Medicine Reports

Article Title: Overexpression of microRNA-155 alleviates palmitate-induced vascular endothelial cell injury in human umbilical vein endothelial cells by negatively regulating the Wnt signaling pathway

doi: 10.3892/mmr.2019.10623

Figure Lengend Snippet: Effect of miR-155 on the survival and migration of palmitate-induced human umbilical vein endothelial cells through the regulation of the Wnt signaling pathway. (A) The MTT assay was used to detect the survival of cells in each treatment group at 24, 48 and 72 h. (B) The Transwell assay was used to determine cell migration ability. The Wnt signaling pathway was inhibited using XAV939. The data are presented as the mean ± SD from three independent experiments. *P<0.05 vs. the control group, # P<0.05 vs. the palmitate group, & P<0.05 vs. the mimic + palmitate group, ▲ P<0.05 vs. the antagomir + palmitate group.

Article Snippet: The cell were cultured for a further 18 h at which point 0.1 mM palmitate (Sigma-Aldrich; Merck KGaA) or 4 μM Wnt signaling pathway inhibitor XAV939 (Tocris Bioscience) were added to the culture for the following 24 h. The HUVECs were divided into six groups: Control group (normal cultured HUVECs); palmitate group (treated with 0.1 mM palmitate for 24 h); mimic + palmitate group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); mimic + palmitate + XAV939 group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate and 4 mM XAV939 for 24 h); antagomir + palmitate group (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); antagomir + palmitate + XAV939 (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate and 4 μM XAV939 for 24 h).

Techniques: Migration, MTT Assay, Transwell Assay, Control

Journal: Molecular Medicine Reports

Article Title: Overexpression of microRNA-155 alleviates palmitate-induced vascular endothelial cell injury in human umbilical vein endothelial cells by negatively regulating the Wnt signaling pathway

doi: 10.3892/mmr.2019.10623

Figure Lengend Snippet: miR-155 affects the level of apoptosis in palmitate-induced human umbilical vein endothelial cells by regulating the Wnt signaling pathway. (A) Levels of apoptosis were detected by flow cytometry. (B) Quantification of the rate of apoptosis. The Wnt signaling pathway was inhibited using XAV939. The data are presented as the mean ± SD from three independent experiments. *P<0.05 vs. the control group, # P<0.05 vs. the palmitate group, & P<0.05 vs. the mimic + palmitate group, ▲ P<0.05 vs. the antagomir+ palmitate group. PI, propidium iodide.

Article Snippet: The cell were cultured for a further 18 h at which point 0.1 mM palmitate (Sigma-Aldrich; Merck KGaA) or 4 μM Wnt signaling pathway inhibitor XAV939 (Tocris Bioscience) were added to the culture for the following 24 h. The HUVECs were divided into six groups: Control group (normal cultured HUVECs); palmitate group (treated with 0.1 mM palmitate for 24 h); mimic + palmitate group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); mimic + palmitate + XAV939 group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate and 4 mM XAV939 for 24 h); antagomir + palmitate group (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); antagomir + palmitate + XAV939 (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate and 4 μM XAV939 for 24 h).

Techniques: Flow Cytometry, Control

Journal: Molecular Medicine Reports

Article Title: Overexpression of microRNA-155 alleviates palmitate-induced vascular endothelial cell injury in human umbilical vein endothelial cells by negatively regulating the Wnt signaling pathway

doi: 10.3892/mmr.2019.10623

Figure Lengend Snippet: Overexpression of miR-155 reduces the levels of ROS by suppressing the Wnt signaling pathway. The Wnt signaling pathway was inhibited using XAV939. The data are presented as the mean ± SD from three independent experiments. *P<0.05 vs. the control group, # P<0.05 vs. the palmitate group, & P<0.05 vs. the mimic + palmitate group, ▲ P<0.05 vs. the antagomir + palmitate group. ROS, reactive oxygen species.

Article Snippet: The cell were cultured for a further 18 h at which point 0.1 mM palmitate (Sigma-Aldrich; Merck KGaA) or 4 μM Wnt signaling pathway inhibitor XAV939 (Tocris Bioscience) were added to the culture for the following 24 h. The HUVECs were divided into six groups: Control group (normal cultured HUVECs); palmitate group (treated with 0.1 mM palmitate for 24 h); mimic + palmitate group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); mimic + palmitate + XAV939 group (transfected with miR-155 mimic for 24 h, followed by treatment with 0.1 mM palmitate and 4 mM XAV939 for 24 h); antagomir + palmitate group (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate for 24 h); antagomir + palmitate + XAV939 (transfected with miR-155 antagomir for 24 h, followed by treatment with 0.1 mM palmitate and 4 μM XAV939 for 24 h).

Techniques: Over Expression, Control